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IGVFFI6590TUUT

released
validation exempted
File Set
IGVFDS4649YECB(mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI6736AYSD
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR2_S19
md5sum
4b937b2133224e7fafa67267d31e4e47
Content MD5sum
d4fc2dca711e8762ea873d908c61473a
File Size
902 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.Auxin0hrs-FF2-BinA-PCR2_S19.yaml
Checkfiles Version
No version due to exemption
Attribution
Jesse Engreitz, Stanford

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Read Names
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
CATAGCGA:CTCT
10 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library