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IGVFFI6558VOZQ

released
validated
File Set
IGVFDS1630KVRE(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI0388ZLUI
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR2_S57
md5sum
12828dd9ee3766288b36a7f9aca27dac
Content MD5sum
95a90a8a8ade8fd15dfa7ae07b037c8f
File Size
903 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.Auxin6hrs-FF1-BinC-PCR2_S57.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
ACAGGTAT:CTCT
8.6 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford