IGVFFI5603KQOW

released
validated
1{
2    "@context": "/terms/",
3    "@id": "/configuration-files/IGVFFI5603KQOW/",
4    "@type": [
5        "ConfigurationFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI5603KQOW",
10    "aliases": [
11        "jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_seqlane2_exprs_seqspec"
12    ],
13    "assay_titles": [
14        "scCRISPR screen"
15    ],
16    "audit": {},
17    "award": {
18        "@id": "/awards/HG011966/",
19        "component": "functional characterization"
20    },
21    "checkfiles_version": "v20",
22    "content_md5sum": "c5c77880879ef9e121d1afa58848c194",
23    "content_type": "seqspec",
24    "creation_timestamp": "2025-04-15T22:47:03.689757+00:00",
25    "derived_manually": false,
26    "file_format": "yaml",
27    "file_set": {
28        "@id": "/measurement-sets/IGVFDS0508ERUQ/",
29        "@type": [
30            "MeasurementSet",
31            "FileSet",
32            "Item"
33        ],
34        "accession": "IGVFDS0508ERUQ",
35        "assay_term": {
36            "@id": "/assay-terms/OBI_0003660/",
37            "term_name": "in vitro CRISPR screen using single-cell RNA-seq"
38        },
39        "data_use_limitation_summaries": [
40            "no certificate"
41        ],
42        "file_set_type": "experimental data",
43        "samples": [
44            {
45                "@id": "/in-vitro-systems/IGVFSM9542NUGS/",
46                "@type": [
47                    "InVitroSystem",
48                    "Biosample",
49                    "Sample",
50                    "Item"
51                ],
52                "accession": "IGVFSM9542NUGS",
53                "classifications": [
54                    "cell line"
55                ],
56                "modifications": [
57                    {
58                        "@id": "/crispr-modifications/8b61bce4-105c-435e-9b32-1c8903566a9b/",
59                        "modality": "activation",
60                        "status": "released",
61                        "summary": "CRISPRa Sp-dCas9-VP64-p65-Rta (VPR)"
62                    }
63                ],
64                "sample_terms": [
65                    {
66                        "@id": "/sample-terms/EFO_0002067/",
67                        "status": "released",
68                        "term_name": "K562"
69                    }
70                ],
71                "status": "released",
72                "summary": "Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
73                "taxa": "Homo sapiens"
74            }
75        ],
76        "status": "released",
77        "summary": "scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
78    },
79    "file_size": 1265,
80    "gene_list_for": [],
81    "href": "/configuration-files/IGVFFI5603KQOW/@@download/IGVFFI5603KQOW.yaml.gz",
82    "input_file_for": [],
83    "integrated_in": [],
84    "lab": {
85        "@id": "/labs/jay-shendure/",
86        "title": "Jay Shendure, UW"
87    },
88    "loci_list_for": [],
89    "md5sum": "1b429bd9236bd47d28a36629709cf8e0",
90    "quality_metrics": [],
91    "release_timestamp": "2025-05-20T16:22:32.685782+00:00",
92    "s3_uri": "s3://igvf-files/2025/04/15/f4f5bc68-9dfe-4185-8674-fc50402c356f/IGVFFI5603KQOW.yaml.gz",
93    "schema_version": "8",
94    "seqspec_of": [
95        "/sequence-files/IGVFFI8600XJSP/",
96        "/sequence-files/IGVFFI2492BPYV/",
97        "/sequence-files/IGVFFI0662BBNP/"
98    ],
99    "status": "released",
100    "submitted_by": {
101        "@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
102        "title": "Beth Martin"
103    },
104    "submitted_file_name": "/net/shendure/vol1/home/martin91/IGVF/forupload/seqspecs/CRISPRa/CRISPRa_IGVFDS0508ERUQ_2_exprs_seqspec_v3.yaml.gz",
105    "summary": "seqspec of IGVFFI8600XJSP, IGVFFI2492BPYV, IGVFFI0662BBNP",
106    "upload_status": "validated",
107    "uuid": "f4f5bc68-9dfe-4185-8674-fc50402c356f",
108    "validate_onlist_files": true
109}