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IGVFFI5214MZXB

released
validated
File Set
IGVFDS2805FQAD(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI8310ZXDQ
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1
md5sum
d675708dcdb97d2e17134dcc5d2f20c0
Content MD5sum
5b09e4356442badc0693b9ec0cc4b8b9
File Size
912 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
CTACCAGG:TGCG
26 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford