{
"@context": "/terms/",
"@id": "/configuration-files/IGVFFI5181NANQ/",
"@type": [
"ConfigurationFile",
"File",
"Item"
],
"accession": "IGVFFI5181NANQ",
"aliases": [
"ansuman-satpathy:seqspec_igvf_exp10_multi_10x2_NGS1"
],
"assay_titles": [
"10x multiome with MULTI-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG012076/",
"component": "mapping"
},
"checkfiles_version": "v16",
"content_md5sum": "9121b92640a00be6a419b5a4adefe4b7",
"content_type": "seqspec",
"creation_timestamp": "2024-09-18T02:21:57.832188+00:00",
"file_format": "yaml",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS6521MNHG/",
"accession": "IGVFDS6521MNHG",
"file_set_type": "lipid-conjugated oligo sequencing",
"samples": [
{
"@id": "/multiplexed-samples/IGVFSM0539NUPM/",
"accession": "IGVFSM0539NUPM",
"classifications": [
"cell line",
"multiplexed sample"
],
"disease_terms": [],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
},
{
"@id": "/sample-terms/EFO_0002067/",
"status": "released",
"term_name": "K562"
},
{
"@id": "/sample-terms/EFO_0002796/",
"status": "released",
"term_name": "Jurkat"
},
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"purpose": "stimulation",
"status": "released",
"summary": "Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours",
"treatment_term_name": "phorbol 13-acetate 12-myristate"
},
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"purpose": "stimulation",
"status": "released",
"summary": "Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
}
]
}
],
"status": "released",
"summary": "lipid-conjugated oligo sequencing for interference single-nucleus ATAC-seq (10x multiome with MULTI-seq), interference single-nucleus RNA sequencing assay (10x multiome with MULTI-seq)"
},
"file_size": 1336,
"gene_list_for": [],
"href": "/configuration-files/IGVFFI5181NANQ/@@download/IGVFFI5181NANQ.yaml.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/ansuman-satpathy/",
"title": "Ansuman Satpathy, Stanford"
},
"loci_list_for": [],
"md5sum": "f5c6f683e3308386b6900cc088fb9224",
"quality_metrics": [],
"release_timestamp": "2025-03-07T01:13:48.770069+00:00",
"s3_uri": "s3://igvf-files/2024/09/18/954882dd-45af-4b81-abbe-a1c8e1efcd14/IGVFFI5181NANQ.yaml.gz",
"schema_version": "7",
"seqspec_of": [
"/sequence-files/IGVFFI2629AQFQ/",
"/sequence-files/IGVFFI6476FJVC/",
"/sequence-files/IGVFFI6133ZBWA/",
"/sequence-files/IGVFFI6902ZMDQ/"
],
"status": "released",
"submitted_by": {
"@id": "/users/353655bc-31a1-4cd9-bac3-c7d34faf722f/",
"title": "Christopher Mcginnis"
},
"submitted_file_name": "/home/users/cmcginni/igvf_utils_out/seqspec/igvf_exp10_multi_10x2_NGS1_multi_seqspec.yaml.gz",
"summary": "seqspec of IGVFFI2629AQFQ, IGVFFI6476FJVC, IGVFFI6133ZBWA, IGVFFI6902ZMDQ",
"upload_status": "validated",
"uuid": "954882dd-45af-4b81-abbe-a1c8e1efcd14",
"validate_onlist_files": false
}