IGVFFI4842GDBV

released
validated
1{
2    "@context": "/terms/",
3    "@id": "/configuration-files/IGVFFI4842GDBV/",
4    "@type": [
5        "ConfigurationFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI4842GDBV",
10    "aliases": [
11        "jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane1_seqlane2_CS1_seqspec"
12    ],
13    "assay_titles": [
14        "scCRISPR screen"
15    ],
16    "audit": {},
17    "award": {
18        "@id": "/awards/HG011966/",
19        "component": "functional characterization"
20    },
21    "checkfiles_version": "v20",
22    "content_md5sum": "98ff18eff02e211473336bc816492015",
23    "content_type": "seqspec",
24    "creation_timestamp": "2025-04-15T22:46:57.338299+00:00",
25    "derived_manually": false,
26    "file_format": "yaml",
27    "file_set": {
28        "@id": "/auxiliary-sets/IGVFDS4621HUKP/",
29        "@type": [
30            "AuxiliarySet",
31            "FileSet",
32            "Item"
33        ],
34        "accession": "IGVFDS4621HUKP",
35        "data_use_limitation_summaries": [
36            "no certificate"
37        ],
38        "file_set_type": "gRNA sequencing",
39        "samples": [
40            {
41                "@id": "/in-vitro-systems/IGVFSM1594ZOQH/",
42                "@type": [
43                    "InVitroSystem",
44                    "Biosample",
45                    "Sample",
46                    "Item"
47                ],
48                "accession": "IGVFSM1594ZOQH",
49                "classifications": [
50                    "cell line"
51                ],
52                "modifications": [
53                    {
54                        "@id": "/crispr-modifications/8b61bce4-105c-435e-9b32-1c8903566a9b/",
55                        "modality": "activation",
56                        "status": "released",
57                        "summary": "CRISPRa Sp-dCas9-VP64-p65-Rta (VPR)"
58                    }
59                ],
60                "sample_terms": [
61                    {
62                        "@id": "/sample-terms/EFO_0002067/",
63                        "status": "released",
64                        "term_name": "K562"
65                    }
66                ],
67                "status": "released",
68                "summary": "Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
69                "taxa": "Homo sapiens"
70            }
71        ],
72        "status": "released",
73        "summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
74    },
75    "file_size": 1421,
76    "gene_list_for": [],
77    "href": "/configuration-files/IGVFFI4842GDBV/@@download/IGVFFI4842GDBV.yaml.gz",
78    "input_file_for": [],
79    "integrated_in": [],
80    "lab": {
81        "@id": "/labs/jay-shendure/",
82        "title": "Jay Shendure, UW"
83    },
84    "loci_list_for": [],
85    "md5sum": "b3616b3e431ac3b402a245acdbb9f904",
86    "quality_metrics": [],
87    "release_timestamp": "2025-05-20T16:22:44.416921+00:00",
88    "s3_uri": "s3://igvf-files/2025/04/15/b9182daf-157c-4ed9-a2da-d2173cbb25bc/IGVFFI4842GDBV.yaml.gz",
89    "schema_version": "8",
90    "seqspec_of": [
91        "/sequence-files/IGVFFI7439CGLC/",
92        "/sequence-files/IGVFFI1163CNBZ/",
93        "/sequence-files/IGVFFI2138EWHH/"
94    ],
95    "status": "released",
96    "submitted_by": {
97        "@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
98        "title": "Beth Martin"
99    },
100    "submitted_file_name": "/net/shendure/vol1/home/martin91/IGVF/forupload/seqspecs/CRISPRa/CRISPRa_IGVFDS4621HUKP_2_CS1_seqspec_v3.yaml.gz",
101    "summary": "seqspec of IGVFFI7439CGLC, IGVFFI1163CNBZ, IGVFFI2138EWHH",
102    "upload_status": "validated",
103    "uuid": "b9182daf-157c-4ed9-a2da-d2173cbb25bc",
104    "validate_onlist_files": false
105}