Home

IGVFFI4790WUCA

released
validated
File Set
IGVFDS7242NZGL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)
Summary
seqspec of IGVFFI3891DAKN
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR2_S171
md5sum
fd53169792fe51cc4256737d9d519f8c
Content MD5sum
3c3ae3234062a13adbb667d1bc72fab9
File Size
908 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1-Auxin0hrs-FF1-BinC-PCR2_S171.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
GAAGGAAG:CATA
6.9 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford