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IGVFFI4747OOCN

released
validated
File Set
IGVFDS4521RIQF(CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
seqspec of IGVFFI7195IFIQ, IGVFFI3023HOII
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:hPA-Seqspec-perturbseq-10xlane4-GEX-L2
md5sum
a8bfcbb87e5e995cf46f69284ebd2d67
Content MD5sum
bd10a14b4d44f5ba0ba381af7d9a9fc8
File Size
1.3 KB
Submitted File Name
/work/rr151/SamAIM2neuro_data/AstrocytehPA-Seqspec-10xlane4-GEX-L2.yaml.gz
Checkfiles Version
v20
Attribution
Charles Gersbach, Duke

seqspec File Of

2 items
Read Names
fastq
reads
4
Read 2
2
9.3 GB
Charles Gersbach, Duke
validated
fastq
reads
4
Read 1
2
3.8 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library