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IGVFFI4640NSBO
IGVFFI4640NSBO
released
validated
Json view
File Set
IGVFDS0591VNKJ
(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI9916XQDH, IGVFFI2856WURS
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep3-rep3-fastq-lane1_seqspec
md5sum
9e27222921c561febc0d0feaaa7de8bf
Content MD5sum
7faaad6b2c8e8ad89724a281feb2a60a
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD3_S3nS9_L001.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2856WURS
fastq
reads
3
Illumina NovaSeq 6000
HMCCWDSX3
1
4.1 GB
Charles Gersbach, Duke
validated
IGVFFI9916XQDH
fastq
reads
3
Illumina NovaSeq 6000
HMCCWDSX3
1
2.3 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM5189UBOI
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke