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INDEXED
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IGVFFI4432MMHG
IGVFFI4432MMHG
released
validated
Json view
File Set
IGVFDS2399UCGA
(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI2639BDZX, IGVFFI4948ANCU
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep2-rep2-fastq-lane4_seqspec
md5sum
23a92998312e918081c2f3206778d4b2
Content MD5sum
62e6ad8d771e423c548de1859528e912
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD2_S2nS8_L004.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2639BDZX
fastq
reads
2
Illumina NovaSeq 6000
HMCCWDSX3
4
3.8 GB
Charles Gersbach, Duke
validated
IGVFFI4948ANCU
fastq
reads
2
Illumina NovaSeq 6000
HMCCWDSX3
4
2.2 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2747RJFY
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke