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IGVFFI3935RVGM

released
validated
File Set
IGVFDS7160FCCA(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
seqspec of IGVFFI1449FMDD, IGVFFI2664QRZC
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:hPA-Seqspec-perturbseq-10xlane4-CRISPR-L2
md5sum
933b303266f1f0fe7ce2090628004af7
Content MD5sum
d9ee31246f4792d8261df1f928bdeba5
File Size
1.4 KB
Submitted File Name
/work/rr151/SamAIM2neuro_data/AstrocytehPA-Seqspec-10xlane4-crispr-L2.yaml.gz
Checkfiles Version
v20
Attribution
Charles Gersbach, Duke

seqspec File Of

2 items
Read Names
fastq
reads
4
Read 1
2
929 MB
Charles Gersbach, Duke
validated
fastq
reads
4
Read 2
2
1.3 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library