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IGVFFI3933DRIM
IGVFFI3933DRIM
released
validated
Json view
File Set
IGVFDS9108NLTS
(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI9294QFQU, IGVFFI3564HSSU
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep1-rep1-fastq-lane2_seqspec
md5sum
fe917e4a5232e5ff99c17e40e553a693
Content MD5sum
9d0eae692777e709e03a8caefbd21a70
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD1_S4nS10_L002.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI3564HSSU
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
2
1.4 GB
Charles Gersbach, Duke
validated
IGVFFI9294QFQU
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
2
2.6 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM8036VPRU
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke