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IGVFFI3571CWKX
IGVFFI3571CWKX
released
validated
Json view
File Set
IGVFDS9813VHFD
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI7019QDVE, IGVFFI7645VURG
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep1-rep1-fastq-lane2_seqspec
md5sum
02a491e773aa28afaad37f85a613da08
Content MD5sum
4d4bddb85f3c612eb4ac967d64a24b72
File Size
982 B
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD1_S1nS7_L002.rna.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI7019QDVE
fastq
reads
1
Illumina NovaSeq 6000
2
7.0 GB
Charles Gersbach, Duke
validated
IGVFFI7645VURG
fastq
reads
1
Illumina NovaSeq 6000
2
17 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM3795JDHP
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke