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IGVFFI3025IEAV

released
validated
File Set
IGVFDS7994GDON(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
seqspec of IGVFFI8354OSIZ, IGVFFI5890JKQC
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:hPA-Seqspec-perturbseq-10xlane1-CRISPR-L2
md5sum
69de27c3e52bc84154958fb35228b86e
Content MD5sum
db2ad3596e4ccf3558e5328276dc648b
File Size
1.4 KB
Submitted File Name
/work/rr151/SamAIM2neuro_data/AstrocytehPA-Seqspec-10xlane1-crispr-L2.yaml.gz
Checkfiles Version
v20
Attribution
Charles Gersbach, Duke

seqspec File Of

2 items
Read Names
fastq
reads
4
Read 2
2
1.4 GB
Charles Gersbach, Duke
validated
fastq
reads
4
Read 1
2
1.0 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library