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IGVFFI2907BHMS
IGVFFI2907BHMS
released
validated
Json view
File Set
IGVFDS3945FAEL
(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI8543ICNA, IGVFFI2756XAJA
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep3-rep3-fastq-lane4_seqspec
md5sum
1d589637006e3e8523d66ff7e6362d5f
Content MD5sum
45d168f2beff6eed3bf9155b60fafc90
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD3_S6nS12_L004.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2756XAJA
fastq
reads
3
Illumina NovaSeq 6000
HMCCWDSX3
4
1.3 GB
Charles Gersbach, Duke
validated
IGVFFI8543ICNA
fastq
reads
3
Illumina NovaSeq 6000
HMCCWDSX3
4
2.5 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2105CLXS
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke