IGVFFI2564WQBV

released
validated
File Set
IGVFDS6870QBVM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI3227NNAQ
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_SSFA2-Auxin0hrs-FF2-BinC-PCR3-Rep1_S69
md5sum
cb2aed9da84f80bd62598920816c1a27
Content MD5sum
8adc9e8b3415e845b7350bc55c286f8e
File Size
912 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.SSFA2-Auxin0hrs-FF2-BinC-PCR3-Rep1_S69.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
TCGGAATG:GCCA
21 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Attribution