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IGVFFI2184ODYQ
IGVFFI2184ODYQ
released
validated
Json view
File Set
IGVFDS1333MBPK
(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI2557GAGA, IGVFFI5242KCVA
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep2-rep2-fastq-lane1_seqspec
md5sum
e516b2f4d510bafd450e494a41832422
Content MD5sum
3ea337cc508c252129ba5a62fca59620
File Size
982 B
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD2_S2nS8_L001.rna.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2557GAGA
fastq
reads
2
Illumina NovaSeq 6000
1
6.6 GB
Charles Gersbach, Duke
validated
IGVFFI5242KCVA
fastq
reads
2
Illumina NovaSeq 6000
1
17 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2747RJFY
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke