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IGVFFI2071NCQF
IGVFFI2071NCQF
released
validated
Json view
File Set
IGVFDS8029DSPH
(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI4222KPGA, IGVFFI7250XPAW
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep1-rep1-fastq-lane3_seqspec
md5sum
3f0e446568dfed4951bae219b4944a79
Content MD5sum
45621f99fdf3ed1dcf8c87d9902a24c0
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRiD1_S1nS7_L003.crispr.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI4222KPGA
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
3
2.5 GB
Charles Gersbach, Duke
validated
IGVFFI7250XPAW
fastq
reads
1
Illumina NovaSeq 6000
HMCCWDSX3
3
4.4 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM3795JDHP
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke