IGVFFI1763IVWB

released
validated
File Set
IGVFDS7160FCCA(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
seqspec of IGVFFI5902VIRS, IGVFFI2728ZPGG
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:hPA-Seqspec-perturbseq-10xlane4-CRISPR-L1
md5sum
211fd8d1f3269da5f0ae6659e4b7c715
Content MD5sum
9917abbe0716a2ed0ce6f3bf86d48570
File Size
1.4 KB
Submitted File Name
/work/rr151/SamAIM2neuro_data/AstrocytehPA-Seqspec-10xlane4-crispr-L1.yaml.gz
Checkfiles Version
Attribution

seqspec File Of

2 items
Read Names
fastq
reads
4
Read 2
1
1.4 GB
Charles Gersbach, Duke
validated
fastq
reads
4
Read 1
1
945 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library