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INDEXED
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IGVFFI1530GXXJ
IGVFFI1530GXXJ
released
validated
Json view
File Set
IGVFDS1675WFHV
(CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI5263FBFP, IGVFFI2784HYOZ
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep2-rep2-fastq-lane2_seqspec
md5sum
9191f5a6019358ed43c9d77a24f6220c
Content MD5sum
d29a1f953872c3ae2f12016c89647cb0
File Size
984 B
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD2_S5nS11_L002.rna.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI2784HYOZ
fastq
reads
2
Illumina NovaSeq 6000
2
16 GB
Charles Gersbach, Duke
validated
IGVFFI5263FBFP
fastq
reads
2
Illumina NovaSeq 6000
2
6.3 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM6223YCQV
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke