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IGVFFI1263XHMB

released
validation exempted
File Set
IGVFDS9498BKLC(mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI8116SREE
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR4_S131
md5sum
08570623c448ca7761e4f37fea5fbda6
Content MD5sum
569cea31c56ad3996c8c2a185c9f8f58
File Size
902 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.Auxin0hrs-FF3-BinE-PCR4_S131.yaml
Checkfiles Version
No version due to exemption
Attribution
Jesse Engreitz, Stanford

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Read Names
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
TTACGCAC:ATTG
20 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library