IGVFFI1168ECOQ

released
validated
File Set
IGVFDS6339VOWW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI0863TIZV
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_FAM3C_Auxin6hrs-FF1-BinE-PCR4-Rep1_S197
md5sum
3e45407e54888d616169072336c65c9c
Content MD5sum
f4e036a290d313e4915de2e206333ccc
File Size
914 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.FAM3C_Auxin6hrs-FF1-BinE-PCR4-Rep1_S197.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
GAACCTAG:ATTG
20 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Attribution