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IGVFFI1109UFAS

released
validated
File Set
IGVFDS8375BGSZ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)
Summary
seqspec of IGVFFI3757DBEL
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR2_S170
md5sum
79018f72fa379450d8bb84068c858f02
Content MD5sum
4ad86959ab31d21a5768e47a5d6ec2d5
File Size
909 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1-Auxin0hrs-FF1-BinB-PCR2_S170.yaml

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
ATTCCTCT:CATA
6.6 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford