{
"@context": "/terms/",
"@id": "/configuration-files/IGVFFI1001DNEG/",
"@type": [
"ConfigurationFile",
"File",
"Item"
],
"accession": "IGVFFI1001DNEG",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "1adb8e50e42d438f52b29bf5e149c450",
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:46:47.575490+00:00",
"file_format": "yaml",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS4252ROMJ/",
"accession": "IGVFDS4252ROMJ",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM4577TQPO/",
"accession": "IGVFSM4577TQPO",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
"modality": "degradation",
"status": "released",
"summary": "inactive AID system targeting RAD21"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"status": "released",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 911,
"gene_list_for": [],
"href": "/configuration-files/IGVFFI1001DNEG/@@download/IGVFFI1001DNEG.yaml.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"md5sum": "1b6fe007e26d0164eea837849a7528b3",
"release_timestamp": "2024-07-10T20:19:29.329609+00:00",
"s3_uri": "s3://igvf-files/2024/06/11/2a96dad3-e1be-42f5-8569-48b331982192/IGVFFI1001DNEG.yaml.gz",
"schema_version": "7",
"seqspec_of": [
"/sequence-files/IGVFFI1890PKGY/"
],
"status": "released",
"submitted_by": {
"@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70.yaml",
"summary": "seqspec of IGVFFI1890PKGY",
"upload_status": "validated",
"uuid": "2a96dad3-e1be-42f5-8569-48b331982192"
}