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IGVFFI0439ROZG

released
validated
File Set
IGVFDS9108NLTS(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI2004LGCX, IGVFFI9768TMWP
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep1-rep1-fastq-lane1_seqspec
md5sum
540bd40733134a6ee38fac477e5b88ac
Content MD5sum
191e2b6cb99ac19715bb6b9f9816872a
File Size
1.1 KB
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD1_S4nS10_L001.crispr.yaml.gz

seqspec File Of

2 items
fastq
reads
1
HMCCWDSX3
1
2.6 GB
Charles Gersbach, Duke
validated
fastq
reads
1
HMCCWDSX3
1
1.4 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke