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IGVFDS8349EMBV

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep1_S113
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep2_S168
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep1_S119
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep2_S174
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep1_S125
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep2_S180
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep1_S131
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep2_S186

Sequencing Results

8 items
fastq
reads
1
Illumina HiSeq 2500
ACCAACTG:TTGA
28 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
ACCAACTG:GCCA
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TGTCGGAT:TTGA
29 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TCGGAATG:TTGA
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TGTCGGAT:GCCA
24 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
GCACATCT:TTGA
24 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
TCGGAATG:GCCA
26 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
GCACATCT:GCCA
17 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep1_S113
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR1_Rep2_S168
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep1_S119
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR2_Rep2_S174
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep1_S125
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR3_Rep2_S180
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep1_S131
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinC-PCR4_Rep2_S186
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford