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IGVFDS6541EVXL

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep1_S28
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep2_S83
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep1_S34
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep2_S101

Sequencing Results

8 items
fastq
reads
1
Illumina HiSeq 2500
GTCTGATG:TCTC
31 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
ATTGTCTG:TGCG
49 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCTATGCC:TGCG
42 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCAGAGCT:TGCG
37 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCAGAGCT:TCTC
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
ATTGTCTG:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
GTCTGATG:TGCG
38 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
Illumina HiSeq 2500
CCTATGCC:TCTC
6.6 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep1_S28
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep2_S83
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep1_S34
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep2_S101
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford