{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS6541EVXL/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS6541EVXL",
"aliases": [
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep1_S28",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR1_Rep2_S83",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep1_S34",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep2_S101"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Auxiliary set [IGVFDS6541EVXL](/auxiliary-sets/IGVFDS6541EVXL/) has sequence file(s) [IGVFFI1007CYRL](/sequence-files/IGVFFI1007CYRL/), [IGVFFI3864GYTT](/sequence-files/IGVFFI3864GYTT/), [IGVFFI0409CMRD](/sequence-files/IGVFFI0409CMRD/), [IGVFFI8915JKET](/sequence-files/IGVFFI8915JKET/), [IGVFFI8115RHRN](/sequence-files/IGVFFI8115RHRN/), [IGVFFI0584UVTC](/sequence-files/IGVFFI0584UVTC/), [IGVFFI2004HRIY](/sequence-files/IGVFFI2004HRIY/), [IGVFFI7539GUVK](/sequence-files/IGVFFI7539GUVK/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS6541EVXL/"
}
]
},
"award": {
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"component": "functional characterization",
"contact_pi": {
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"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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"donors": [
{
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"aliases": [
"igvf:donor_of_HCT116"
],
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"status": "released",
"taxa": "Homo sapiens"
}
],
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8915JKET/",
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],
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"creation_timestamp": "2024-06-01T02:01:15.680217+00:00",
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"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI0667ZMOX/",
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8115RHRN/",
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],
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI0584UVTC/",
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],
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},
{
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],
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"upload_status": "validated"
},
{
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],
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},
{
"@id": "/sequence-files/IGVFFI7539GUVK/",
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],
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},
{
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},
{
"@id": "/configuration-files/IGVFFI8671HDLD/",
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},
{
"@id": "/configuration-files/IGVFFI0369MZCG/",
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],
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},
{
"@id": "/configuration-files/IGVFFI2787QCSH/",
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},
{
"@id": "/configuration-files/IGVFFI1437HJWT/",
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],
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"upload_status": "validated"
}
],
"input_for": [
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],
"lab": {
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"title": "Jesse Engreitz, Stanford"
},
"measurement_sets": [
{
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"aliases": [
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR2_Rep2_S89",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40",
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46",
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],
"preferred_assay_title": "CRISPR FlowFISH screen",
"status": "released"
}
],
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"samples": [
{
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"Biosample",
"Sample",
"Item"
],
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"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40",
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"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46",
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],
"classifications": [
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],
"construct_library_sets": [
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],
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{
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},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
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}
],
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{
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"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:00:55.081494+00:00",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "ba77dfea-ddf0-457b-921b-c64a8af04fa6"
}