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IGVFDS3809LQRK

released
File Set Type
cell sorting
Summary
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
Aliases
jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF2

Tabular Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
sample sort parameters
sample sort parameters
Jesse Engreitz, Stanford
489 B
validated

Samples

6 items
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

6 items
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinF-PCR1-Rep1_S42
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinF-PCR2-Rep1_S48
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinF-PCR3-Rep1_S96
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinF-PCR4-Rep1_S102
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR1-Rep1_S40
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR2-Rep1_S46
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR3-Rep1_S94
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinD-PCR4-Rep1_S100
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR1-Rep1_S38
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR2-Rep1_S44
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR3-Rep1_S92
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinB-PCR4-Rep1_S98
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR1-Rep1_S37
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR2-Rep1_S43
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR3-Rep1_S91
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinA-PCR4-Rep1_S97
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinC-PCR1-Rep1_S39
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinC-PCR2-Rep1_S45
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinC-PCR3-Rep1_S93
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinC-PCR4-Rep1_S99
Jesse Engreitz, Stanford
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinE-PCR1-Rep1_S41
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinE-PCR2-Rep1_S47
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinE-PCR3-Rep1_S95
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF2-BinE-PCR4-Rep1_S101
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford