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IGVFDS2532AVSC

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Aliases
jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_CS1
Publications

Sequencing Results (Illumina)

8 items
fastq
reads
R2
1
AAAMCTVHV
1
1.4 GB
Jay Shendure, UW
validated
fastq
reads
I2
1
AAAMCTVHV
2
322 MB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAMCTVHV
1
1.1 GB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAMCTVHV
2
1.0 GB
Jay Shendure, UW
validated
fastq
reads
I2
1
AAAMCTVHV
1
356 MB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAMCTVHV
1
325 MB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAMCTVHV
2
290 MB
Jay Shendure, UW
validated
fastq
reads
R2
1
AAAMCTVHV
2
1.3 GB
Jay Shendure, UW
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_exprs
Jay Shendure, UW

File Sets Using This Auxiliary Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
scCRISPR activation screen: differential gene expression quantifications, sparse gene count matrix
  • jay-shendure:analysis_set_CRISPRa_ipscNeurons
Jay Shendure, UW

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW