{
"@context": "/terms/",
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"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS2532AVSC",
"aliases": [
"jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_CS1"
],
"audit": {
"ERROR": [
{
"category": "inconsistent sequence specifications",
"detail": "Auxiliary set [IGVFDS2532AVSC](/auxiliary-sets/IGVFDS2532AVSC/) has sequence files: [IGVFFI5740EZRO](/sequence-files/IGVFFI5740EZRO/), [IGVFFI5101XXFL](/sequence-files/IGVFFI5101XXFL/), [IGVFFI2904WAAT](/sequence-files/IGVFFI2904WAAT/), [IGVFFI3911GHFM](/sequence-files/IGVFFI3911GHFM/), [IGVFFI7440JFKZ](/sequence-files/IGVFFI7440JFKZ/), [IGVFFI4073XXVS](/sequence-files/IGVFFI4073XXVS/), [IGVFFI8818WFVA](/sequence-files/IGVFFI8818WFVA/), [IGVFFI3761PGKJ](/sequence-files/IGVFFI3761PGKJ/) which share the same `seqspecs` [IGVFFI8632FADI](/configuration-files/IGVFFI8632FADI/), but belong to different sequencing sets. Sequence files in a file set from the same sequencing set are expected to link to the same seqspec file, which should be unique to that sequencing set. A sequencing set is defined by the combination of flowcell ID, lane, and index for non-single cell data, or flowcell ID, lane, index, and sequencing run for single cell data.",
"level": 60,
"level_name": "ERROR",
"name": "audit_inconsistent_seqspec",
"path": "/auxiliary-sets/IGVFDS2532AVSC/"
}
]
},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/eb543cda-67b7-493c-84eb-187d0dac13ba/",
"title": "Jay Shendure"
},
"title": "Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems"
},
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{
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"status": "released",
"summary": "GRCh38 guide RNA sequences"
}
],
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"summary": "guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2023-12-08T23:52:07.294289+00:00",
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{
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"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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],
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"upload_status": "validated"
},
{
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],
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},
{
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"upload_status": "validated"
},
{
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],
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},
{
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"status": "released",
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},
{
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],
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},
{
"@id": "/sequence-files/IGVFFI8818WFVA/",
"accession": "IGVFFI8818WFVA",
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],
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},
{
"@id": "/sequence-files/IGVFFI3761PGKJ/",
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],
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},
{
"@id": "/configuration-files/IGVFFI8632FADI/",
"accession": "IGVFFI8632FADI",
"aliases": [
"jay-shendure:seqspec_of_set_IGVFDS2532AVSC"
],
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"creation_timestamp": "2025-04-02T18:30:07.535587+00:00",
"file_format": "yaml",
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"status": "in progress",
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"upload_status": "validated"
}
],
"input_for": [
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],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"measurement_sets": [
{
"@id": "/measurement-sets/IGVFDS2385QENN/",
"accession": "IGVFDS2385QENN",
"aliases": [
"jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_exprs"
],
"preferred_assay_title": "scCRISPR screen",
"status": "released"
}
],
"publications": [
{
"@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
"publication_identifiers": [
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],
"status": "released"
}
],
"release_timestamp": "2024-08-13T20:47:22.719369+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM8317ZTFV/",
"@type": [
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"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8317ZTFV",
"aliases": [
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],
"cellular_sub_pool": "10Xlane3",
"classifications": [
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],
"institutional_certificates": [],
"modifications": [
{
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"modality": "activation",
"status": "released"
}
],
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{
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"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:WTC11"
],
"status": "released",
"summary": "GM25256 (WTC-11)",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "released",
"summary": "Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library",
"targeted_sample_term": {
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"status": "released",
"term_name": "neuron"
},
"taxa": "Homo sapiens"
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_files_timestamp": "2023-12-09T00:51:13.101456+00:00",
"summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci",
"uuid": "d6a27c6b-d621-4087-8026-03e8c25dc7b3"
}