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IGVFDS2372JFBI

released
File Set Type
gRNA sequencing
Summary
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Aliases
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189

Sequencing Results

8 items
fastq
reads
1
TACTTAGC:GCCA
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTCGCTGA:GCCA
26 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCTGGCGA:TTGA
18 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TACTTAGC:TTGA
23 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTCGCTGA:TTGA
31 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGTTGCTT:GCCA
26 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AGTTGCTT:TTGA
24 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCTGGCGA:GCCA
20 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Related Measurement Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford