{
"@context": "/terms/",
"@id": "/auxiliary-sets/IGVFDS2372JFBI/",
"@type": [
"AuxiliarySet",
"FileSet",
"Item"
],
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"aliases": [
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134",
"jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Auxiliary set [IGVFDS2372JFBI](/auxiliary-sets/IGVFDS2372JFBI/) has sequence file(s) [IGVFFI7923UEBL](/sequence-files/IGVFFI7923UEBL/), [IGVFFI7683TXPK](/sequence-files/IGVFFI7683TXPK/), [IGVFFI5849CCCH](/sequence-files/IGVFFI5849CCCH/), [IGVFFI3283FABB](/sequence-files/IGVFFI3283FABB/), [IGVFFI1366IKMD](/sequence-files/IGVFFI1366IKMD/), [IGVFFI1641ICFG](/sequence-files/IGVFFI1641ICFG/), [IGVFFI9101IEYD](/sequence-files/IGVFFI9101IEYD/), [IGVFFI3695WJWY](/sequence-files/IGVFFI3695WJWY/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/auxiliary-sets/IGVFDS2372JFBI/"
}
]
},
"award": {
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"component": "functional characterization",
"contact_pi": {
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"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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"aliases": [
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],
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"taxa": "Homo sapiens"
}
],
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],
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},
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],
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},
{
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],
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},
{
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],
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"upload_status": "validated"
},
{
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],
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"upload_status": "validated"
},
{
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],
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},
{
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],
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},
{
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},
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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],
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}
],
"input_for": [
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],
"lab": {
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"title": "Jesse Engreitz, Stanford"
},
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{
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183",
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"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189"
],
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}
],
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{
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"Biosample",
"Sample",
"Item"
],
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"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134",
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],
"classifications": [
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],
"construct_library_sets": [
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],
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{
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},
{
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}
],
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{
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"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"status": "released",
"treatment_term_name": "5-Phenyl-1H-indole-3-acetic acid"
}
]
}
],
"schema_version": "12",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:03:51.825076+00:00",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"uuid": "2e75aa19-c7b1-4773-bb78-d101c2bc4802"
}