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IGVFDS9192YMGI

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Aliases
gary-hon:CM_Pilot_2_PB_RNA
File Set Type
principal analysis
Summary
CRISPR interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix
Workflows
Samples
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library
Protocols
Attribution
Gary Hon, UT

Files

3 items
h5ad
sparse gene count matrix
cell by gene expression in sparse gene count matrix
Gary Hon, UT
19 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
783 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
4.5 GB
validated

File Association Graph

IGVFDS8701ERXN4 filesexperimental data4 filesIGVFFI8111GDGEpklraw feature barcode…IGVFFI3575TVLQh5adsparse gene count matrixIGVFFI7202EABOhdf5filtered feature…IGVFDS6402AFPQ8 filescell hashing barcode…8 filesIGVFDS7019VPKV8 filesgRNA sequencing8 filesIGVFDS7242KAVQ8 filesgRNA sequencing8 filesIGVFDS9976WMCI8 filesgRNA sequencing8 filesIGVFDS3212YIVB8 filesgRNA sequencing8 filesIGVFDS2136RFQE8 filesgRNA sequencing8 filesIGVFDS0890CUXC4 filesexperimental data4 filesIGVFDS1726RJYG8 filescell hashing barcode…8 filesIGVFDS9925GTMC8 filescell hashing barcode…8 filesIGVFDS2406OSFC8 filescell hashing barcode…8 filesIGVFDS1663ZHVE4 filesexperimental data4 filesIGVFDS2593KURF4 filesexperimental data4 filesIGVFDS9005AVSB4 filesexperimental data4 filesIGVFDS1334EIHS4 filesexperimental data4 filesIGVFDS7504HTXW8 filesgRNA sequencing8 filesIGVFDS0588CNDW8 filescell hashing barcode…8 filesIGVFDS9856AQIW8 filescell hashing barcode…8 filesIGVFDS2333GHSM8 filesgRNA sequencing8 filesIGVFDS4323LXLZ4 filesexperimental data4 filesIGVFDS8202GWXE8 filesgRNA sequencing8 filesIGVFDS8705MRYI4 filesexperimental data4 filesIGVFDS5986XHYV8 filescell hashing barcode…8 filesIGVFDS0019ZVVN8 filescell hashing barcode…8 files
File Set
File
AnalysisSet
AuxiliarySet
MeasurementSet

Samples

8 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

MeasurementSet Input File Sets

8 items
Associated Auxiliary Sets
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

AuxiliarySet Input File Sets

16 items
Associated Measurement Sets
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library