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IGVFDS9143TFRW

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispri-ccr7
File Set Type
principal analysis
Summary
CRISPR interference FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control
Publications
Samples
Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of CCR7
Protocols
Attribution
Charles Gersbach, Duke

Files

6 items
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
70 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
56 MB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
40 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
36 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
9.5 MB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

4 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

2 items
Aliases
  • charles-gersbach:cd8-tcells-donor-20
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-21
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in many genes

MeasurementSet Input File Sets

4 items
Controls
CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)