Home

IGVFDS8839AFQB

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispri-b2m
File Set Type
principal analysis
Summary
CRISPR interference FACS screen targeting B2M: fold change over control, guide quantifications
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of B2M

Files

2 items
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
11 KB
validated
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
17 KB
validated

File Association Graph

Loading…
File Set
File
AnalysisSet

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-19
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-18
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-17
unspecified
Homo sapiens

AnalysisSet Input File Sets

1 item
Accession
Summary
CRISPR interference FACS screen targeting B2M: alignments

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke