IGVFDS8364HJHL

released

Aliases

charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over

File Set Type

principal analysis

Summary

CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Samples

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R

Files

2 items

IGVFFI2629EIOZ

tsv

fold change over control

custom assembly fold change over control

Charles Gersbach, Duke

376 KB

validated

IGVFFI8017PKLD

csv

guide quantifications

custom assembly guide quantifications

Charles Gersbach, Duke

144 KB

validated

File Association Graph

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File Set

File

AnalysisSet

Samples

4 items

Sample Terms

Summary

IGVFSM3895SURE

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7158ADKU

PrimaryCell

CD8-positive, alpha-beta memory T cell

IGVFSM7887TFLH

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM8084QKPW

PrimaryCell

CD8-positive, alpha-beta memory T cell