IGVFDS8174BPPS

{
    "@context": "/terms/",
    "@id": "/analysis-sets/IGVFDS8174BPPS/",
    "@type": [
        "AnalysisSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS8174BPPS",
    "aliases": [
        "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1_Variant-EFFECTS_analysis"
    ],
    "assay_titles": [
        "Variant-EFFECTS"
    ],
    "audit": {
        "ERROR": [
            {
                "category": "upload status not validated",
                "detail": "Tabular file [IGVFFI0706LAPB](/tabular-files/IGVFFI0706LAPB/) has `upload_status` file not found. Files are expected to be validated or validation exempted.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_upload_status",
                "path": "/tabular-files/IGVFFI0706LAPB/"
            },
            {
                "category": "unexpected input file set",
                "detail": "Analysis set [IGVFDS8174BPPS](/analysis-sets/IGVFDS8174BPPS/) links to file set(s): [IGVFDS8210FQOO](/measurement-sets/IGVFDS8210FQOO/) in `input_file_sets` that are not represented in the `derived_from` of the file sets of the files in this analysis. The analysis set input files are expected to belong to the input file sets.",
                "level": 60,
                "level_name": "ERROR",
                "name": "audit_input_file_sets_derived_from",
                "path": "/analysis-sets/IGVFDS8174BPPS/"
            }
        ],
        "NOT_COMPLIANT": [
            {
                "category": "missing analysis step version",
                "detail": "Analysis set [IGVFDS8174BPPS](/analysis-sets/IGVFDS8174BPPS/) links to file(s) [IGVFFI0706LAPB](/tabular-files/IGVFFI0706LAPB/) that are missing `analysis_step_version`. Analysis set files are expected to specify analysis step version.",
                "level": 50,
                "level_name": "NOT_COMPLIANT",
                "name": "audit_analysis_set_files_missing_analysis_step_version",
                "path": "/analysis-sets/IGVFDS8174BPPS/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_for": [],
    "creation_timestamp": "2024-12-10T23:54:32.361679+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "file_set_type": "principal analysis",
    "files": [
        {
            "@id": "/tabular-files/IGVFFI0706LAPB/",
            "accession": "IGVFFI0706LAPB",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1_Variant-EFFECTS_variant_effects_file"
            ],
            "content_type": "variant effects",
            "controlled_access": false,
            "creation_timestamp": "2024-12-17T21:03:56.233093+00:00",
            "file_format": "tsv",
            "href": "/tabular-files/IGVFFI0706LAPB/@@download/IGVFFI0706LAPB.tsv.gz",
            "s3_uri": "s3://igvf-files/2024/12/17/6597a55e-71e1-4fda-a2cd-3637a99701e7/IGVFFI0706LAPB.tsv.gz",
            "status": "in progress",
            "submitted_file_name": "PPIF_5PrimeSpliceIndividual_AGGT_TCAG_AllelicEffectsStats.tsv.gz",
            "upload_status": "file not found"
        }
    ],
    "functional_assay_mechanisms": [],
    "input_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS8210FQOO/",
            "accession": "IGVFDS8210FQOO",
            "aliases": [
                "jesse-engreitz:THP1-PE2_Exp7_PPIF_5SpliceSite-IndividualVariantExp_NegControl_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS1289LJDH/",
            "accession": "IGVFDS1289LJDH",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinA_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS1096VFTG/",
            "accession": "IGVFDS1096VFTG",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinB_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS3617RUHJ/",
            "accession": "IGVFDS3617RUHJ",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinC_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS3152TFYP/",
            "accession": "IGVFDS3152TFYP",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinD_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS7850WHCP/",
            "accession": "IGVFDS7850WHCP",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-FACS_Input_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS6525TINW/",
            "accession": "IGVFDS6525TINW",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinA_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS5799EHEX/",
            "accession": "IGVFDS5799EHEX",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinB_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS5011SBVA/",
            "accession": "IGVFDS5011SBVA",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinC_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS3185QXRT/",
            "accession": "IGVFDS3185QXRT",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinD_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS9135QDMQ/",
            "accession": "IGVFDS9135QDMQ",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-FACS_Input_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS9423AXCY/",
            "accession": "IGVFDS9423AXCY",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinA_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS6052JSPO/",
            "accession": "IGVFDS6052JSPO",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinB_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS3625ICZE/",
            "accession": "IGVFDS3625ICZE",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinC_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS6684QDAK/",
            "accession": "IGVFDS6684QDAK",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinD_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS5663OZRS/",
            "accession": "IGVFDS5663OZRS",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-FACS_Input_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS6981TLKY/",
            "accession": "IGVFDS6981TLKY",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinA_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS2117LVGL/",
            "accession": "IGVFDS2117LVGL",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinB_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS8217QYTZ/",
            "accession": "IGVFDS8217QYTZ",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS6997ZCVM/",
            "accession": "IGVFDS6997ZCVM",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinD_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        },
        {
            "@id": "/measurement-sets/IGVFDS2754BSGV/",
            "accession": "IGVFDS2754BSGV",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-FACS_Input_AmpliconSequencing"
            ],
            "file_set_type": "experimental data",
            "status": "preview"
        }
    ],
    "input_for": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "sample_summary": "THP-1 cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of PPIF",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM7910KYRO/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM7910KYRO",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM4533NOSQ/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4533NOSQ",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-FACS_Input"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM8027FBQH/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM8027FBQH",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-FACS_Input"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM2418RCAK/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM2418RCAK",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM5487NBDD/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM5487NBDD",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM9420YMIN/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9420YMIN",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM1138SBPT/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1138SBPT",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM6214ZWHT/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM6214ZWHT",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-FACS_Input"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                "/construct-library-sets/IGVFDS6555EOZU/"
            ],
            "modifications": [
                {
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            ],
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                {
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                        "SampleTerm",
                        "OntologyTerm",
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            ],
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            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM9431CHMK/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9431CHMK",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinB"
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                "cell line"
            ],
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                "/construct-library-sets/IGVFDS6555EOZU/"
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                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
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                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
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                        "igvf:THP-1"
                    ],
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                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM9629HOBW/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9629HOBW",
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                "jesse-engreitz:THP1-PE2_Exp7_PPIF_5SpliceSite-IndividualVariantExp_NegControl"
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                "cell line"
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                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
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                }
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                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
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                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM5877MLYX/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM5877MLYX",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinD"
            ],
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                "cell line"
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                "/construct-library-sets/IGVFDS6555EOZU/"
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                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
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                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
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                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
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            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM4402MARX/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4402MARX",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinD"
            ],
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                "cell line"
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                "/construct-library-sets/IGVFDS6555EOZU/"
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                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
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            ],
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                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM1364MMFG/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1364MMFG",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinC"
            ],
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                "/construct-library-sets/IGVFDS6555EOZU/"
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                {
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                    "modality": "prime editing",
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            ],
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                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
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                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM3302PLSS/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM3302PLSS",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinA"
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                    "term_name": "THP-1"
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            "taxa": "Homo sapiens"
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        {
            "@id": "/in-vitro-systems/IGVFSM5820IIOC/",
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                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM5820IIOC",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep1-BinC"
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                    "summary": "THP-1",
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            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
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        {
            "@id": "/in-vitro-systems/IGVFSM8728FTOV/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
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            "aliases": [
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            "taxa": "Homo sapiens"
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        {
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                "Biosample",
                "Sample",
                "Item"
            ],
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            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM2884AVNM/",
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                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM2884AVNM",
            "aliases": [
                "jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep2-BinC"
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                    "summary": "THP-1",
                    "term_name": "THP-1"
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            ],
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            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
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        {
            "@id": "/in-vitro-systems/IGVFSM8619CRIV/",
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                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM8619CRIV",
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                        "igvf:THP-1"
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                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
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            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        },
        {
            "@id": "/in-vitro-systems/IGVFSM4036POBD/",
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                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4036POBD",
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        },
        {
            "@id": "/in-vitro-systems/IGVFSM4190EMFX/",
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                "Sample",
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            ],
            "accession": "IGVFSM4190EMFX",
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                    "summary": "THP-1",
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                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "9",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2024-12-17T21:03:56.233093+00:00",
    "summary": "prime editing Variant-EFFECTS targeting PPIF: variant effects",
    "uuid": "9813ba89-1390-41d5-8bfa-be51ade8d128",
    "workflows": []
}