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Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

CRISPR knockout FACS screen targeting IL7R: alignments

Gersbach FACS CRISPR screen workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications

CRISPR FACS screen

Guide (sgRNA) library targeting transcription start sites in many genes

CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

IGVFDS7944XZYE

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IGVFDS7944XZYE

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