Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications

Gersbach FACS CRISPR screen workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

CRISPR activation FACS screen targeting IL2RA: alignments

gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

IGVFDS7196OWBU

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IGVFDS7196OWBU

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