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IGVFDS7196OWBU

released
Aliases
charles-gersbach:jurkats-analysis-set-crispra-vp64x2-il2ra
File Set Type
principal analysis
Summary
CRISPR activation FACS screen targeting IL2RA: alignments, fold change over control, guide quantifications
Workflows
Publications
Samples
Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA
Protocols

Files

8 items
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
17 MB
validated
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
15 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
15 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
20 MB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
9.2 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
19 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
17 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
18 MB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

6 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:jurkat-tcells-donor-5
unspecified
Homo sapiens
  • charles-gersbach:jurkat-tcells-donor-6
unspecified
Homo sapiens
  • charles-gersbach:jurkat-tcells-donor-4
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in IL2RA

MeasurementSet Input File Sets

6 items
Controls
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke