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Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CD2

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

CRISPR interference FACS screen targeting CD2: alignments

Gersbach FACS CRISPR screen workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2

CRISPR interference FACS screen targeting CD2: fold change over control, guide quantifications

CRISPR FACS screen

CD2 molecule

Guide (sgRNA) library targeting transcription start sites in CD2

CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2

IGVFDS5498STRV

Files

Attribution

IGVFDS5498STRV

Files

File Association Graph

Samples

Donors

AuxiliarySet Input File Sets

File Sets Using This Analysis Set as an Input

Attribution