Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

CRISPR activation FACS screen targeting IL2RA: alignments

Gersbach FACS CRISPR screen workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications

CRISPR FACS screen

interleukin 2 receptor subunit alpha

Guide (sgRNA) library targeting transcription start sites in IL2RA

CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

IGVFDS5478QWPN

Files

Attribution

IGVFDS5478QWPN

Files

File Association Graph

Samples

Donors

AuxiliarySet Input File Sets

File Sets Using This Analysis Set as an Input

Attribution