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IGVFDS5239SBBT

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispra
File Set Type
principal analysis
Summary
activation Perturb-seq: differential gene expression quantifications, filtered feature barcode matrix
Workflows
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
Protocols

Files

4 items
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
408 MB
validated
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
336 MB
validated
tsv
differential gene expression quantifications
GRCh38 differential gene expression quantifications
Charles Gersbach, Duke
23 MB
validated
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
310 MB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet
MeasurementSet

Samples

3 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-8
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-7
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-6
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in 24 genes

MeasurementSet Input File Sets

3 items
Associated Auxiliary Sets
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

AuxiliarySet Input File Sets

3 items
Associated Measurement Sets
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke