audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

differential gene expression quantifications

Charles Gersbach, Duke

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

activation Perturb-seq: differential gene expression quantifications

Gersbach Perturb-seq workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

activation Perturb-seq: filtered feature barcode matrix

gene by gene expression by CRISPR guide capture in filtered feature barcode matrix

activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

gRNA sequencing for activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

basic leucine zipper ATF-like transcription factor

basic leucine zipper ATF-like transcription factor 3

basic helix-loop-helix family member e40

cAMP responsive element modulator

eomesodermin

Fli-1 proto-oncogene, ETS transcription factor

forkhead box D2

forkhead box O1

GA binding protein transcription factor subunit alpha

HIC ZBTB transcriptional repressor 1

interferon regulatory factor 2

Jun proto-oncogene, AP-1 transcription factor subunit

MYB proto-oncogene, transcription factor

nuclear factor of activated T cells 3

NFE2 like bZIP transcription factor 1

nuclear receptor subfamily 1 group D member 1

nuclear receptor subfamily 4 group A member 1

POU class 2 homeobox 1

ras responsive element binding protein 1

transcription factor 7 like 1

ZFP1 zinc finger protein

BTB domain and CNC homolog 2

KLF transcription factor 2

DNA methyltransferase 1

Guide (sgRNA) library targeting transcription start sites in 24 genes

GRCh38 differential gene expression quantifications

IGVFDS5239SBBT

Files

Attribution

IGVFDS5239SBBT

Files

File Association Graph

Samples

Donors

AnalysisSet Input File Sets

Attribution