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IGVFDS5218AVPW

released
Aliases
charles-gersbach:jurkats-intermediate-analysis-crispra-vp64x2-il2ra
File Set Type
intermediate analysis
Summary
CRISPR activation FACS screen targeting IL2RA: alignments
Publications
Samples
Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA

Files

6 items
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
17 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
15 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
20 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
19 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
17 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
18 MB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet

Samples

6 items
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:jurkat-tcells-donor-5
unspecified
Homo sapiens
  • charles-gersbach:jurkat-tcells-donor-6
unspecified
Homo sapiens
  • charles-gersbach:jurkat-tcells-donor-4
unspecified
Homo sapiens

AuxiliarySet Input File Sets

6 items
Associated Measurement Sets
Associated Construct Library Sets
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

File Sets Using This Analysis Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications
  • charles-gersbach:jurkats-analysis-set-crispra-vp64x2-il2ra
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke