IGVFDS5157TBYH

released

Aliases

charles-gersbach:cd8-tcells-analysis-set-crispri-cd2

File Set Type

principal analysis

Summary

CRISPR interference FACS screen targeting CD2: fold change over control, guide quantifications

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Samples

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CD2

Files

2 items

IGVFFI3072IFRD

tsv

fold change over control

custom assembly fold change over control

Charles Gersbach, Duke

13 KB

validated

IGVFFI5502YFXX

csv

guide quantifications

custom assembly guide quantifications

Charles Gersbach, Duke

8.1 KB

validated

File Association Graph

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File Set

File

AnalysisSet

Samples

4 items

Sample Terms

Summary

IGVFSM4694MBIW

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6350DJQS

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7030KVXG

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7658WDNM

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)