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IGVFDS5157TBYH

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispri-cd2
File Set Type
principal analysis
Summary
CRISPR interference FACS screen targeting CD2: alignments, fold change over control, guide quantifications
Workflows
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CD2
Protocols

Files

6 items
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
21 MB
validated
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
13 KB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
8.1 KB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
24 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
21 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
19 MB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

4 items
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

2 items
Aliases
  • charles-gersbach:cd8-tcells-donor-16
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-15
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in CD2

MeasurementSet Input File Sets

4 items
Controls
CRISPR interference FACS screen sorted on the expression of CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR interference FACS screen sorted on the expression of CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR interference FACS screen sorted on the expression of CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
low FACS signal CRISPR interference FACS screen sorted on the expression of CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke