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IGVFDS5106XTZH

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome
File Set Type
principal analysis
Summary
CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R

Files

2 items
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
364 KB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
147 KB
validated

File Association Graph

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File Set
File
AnalysisSet

Samples

4 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

2 items
Aliases
  • charles-gersbach:cd8-tcells-donor-23
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-24
unspecified
Homo sapiens

AnalysisSet Input File Sets

1 item
Accession
Summary
CRISPR knockout FACS screen targeting IL7R: alignments

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke