audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CCR7

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Alejandro Barrera

CRISPR activation FACS screen targeting CCR7: alignments

Gersbach FACS CRISPR screen workflow

HG012053

Ian Whaling

charles-gersbach

Gregory Crawford

Tim Reddy

gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

CRISPR activation FACS screen targeting CCR7: fold change over control, guide quantifications

CRISPR FACS screen

Guide (sgRNA) library targeting transcription start sites in many genes

CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

IGVFDS4488GLKL

Files

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IGVFDS4488GLKL

Files

File Association Graph

Samples

Donors

AuxiliarySet Input File Sets

File Sets Using This Analysis Set as an Input

Attribution