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IGVFDS4430NOFL

released
Aliases
charles-gersbach:cd8-tcells-intermediate-analysis-rna-seq-cas-ko-znf217
File Set Type
intermediate analysis
Summary
knockout RNA-seq: transcriptome alignments
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
Protocols

Files

3 items
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.8 GB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
validated
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.3 GB
validated

File Association Graph

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File Set
File
AnalysisSet
MeasurementSet

Samples

3 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-14
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-13
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens

MeasurementSet Input File Sets

3 items
Associated Construct Library Sets
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

File Sets Using This Analysis Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
knockout RNA-seq: fold change over control, transcript quantifications
  • charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-znf217
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke