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IGVFDS4389OUWU

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Aliases
gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis
File Set Type
principal analysis
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Samples
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library, cellular sub pool(s): subpool_LW277, subpool_LW278, subpool_LW279, subpool_LW280
Protocols
Attribution
Gary Hon, UT

Files

5 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
448 MB
validated
h5ad
sparse gene count matrix
cell by gene expression in sparse gene count matrix
Gary Hon, UT
13 GB
validated
csv
unfiltered local differential expression
GRCh38 GENCODE 32 unfiltered local differential expression
Gary Hon, UT
6.8 KB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
395 MB
validated
csv
unfiltered global differential expression
GRCh38 GENCODE 32 unfiltered global differential expression
Gary Hon, UT
734 KB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet
MeasurementSet

Samples

4 items
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

MeasurementSet Input File Sets

4 items
Associated Auxiliary Sets
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

AuxiliarySet Input File Sets

8 items
Associated Measurement Sets
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library