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IGVFDS3751NDQI

released
Aliases
ansuman-satpathy:igvf4_analysis_set_10x2
File Set Type
intermediate analysis
Summary
10x multiome with MULTI-seq: cell hashing barcodes, fragments, index, peaks, raw feature barcode matrix
Workflows
Samples
Homo sapiens multiplexed sample of GM25256 (WTC-11), differentiated with 0.025 ng/mL acetylated low density lipoprotein for 1 day, 100 ng/mL macrophage colony-stimulating factor 1 for 7 days, 20 ng/mL interferon gamma for 1 day, 50 ng/mL interleukin 4 for 1 day
Protocols

Files

5 items
bed
peaks
GRCh38 peaks
Ansuman Satpathy, Stanford
2.0 MB
validation exempted
tsv
cell hashing barcodes
cell hashing barcodes
Ansuman Satpathy, Stanford
1.2 MB
validated
tsv
fragments
fragments
Ansuman Satpathy, Stanford
6.8 GB
validation exempted
tbi
index
index of IGVFFI7268PNWE
Ansuman Satpathy, Stanford
1.9 MB
validated
hdf5
raw feature barcode matrix
cell by gene in raw feature barcode matrix
Ansuman Satpathy, Stanford
233 MB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet
MeasurementSet

Samples

1 item
Accession
Type
Sample Terms
Disease Terms
Associated Construct Library Sets
Summary
MultiplexedSample
multiplexed sample: Homo sapiens (male) GM25256 (WTC-11) cell line treated with 50 ng/mL interleukin 4 for 1 day; Homo sapiens (male) GM25256 (WTC-11) cell line treated with 100 ng/mL macrophage colony-stimulating factor 1 for 7 days; ... and 13 more samples

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

MeasurementSet Input File Sets

2 items
Associated Auxiliary Sets
single-nucleus ATAC-seq (10x multiome with MULTI-seq)
multiplexed sample: Homo sapiens (male) GM25256 (WTC-11) cell line treated with 50 ng/mL interleukin 4 for 1 day; Homo sapiens (male) GM25256 (WTC-11) cell line treated with 100 ng/mL macrophage colony-stimulating factor 1 for 7 days; ... and 13 more samples
single-nucleus RNA sequencing assay (10x multiome with MULTI-seq)
multiplexed sample: Homo sapiens (male) GM25256 (WTC-11) cell line treated with 50 ng/mL interleukin 4 for 1 day; Homo sapiens (male) GM25256 (WTC-11) cell line treated with 100 ng/mL macrophage colony-stimulating factor 1 for 7 days; ... and 13 more samples

AuxiliarySet Input File Sets

1 item
Auxiliary Sets
Measured Samples
Associated Measurement Sets
lipid-conjugated oligo sequencing for single-nucleus RNA sequencing assay (10x multiome with MULTI-seq), single-nucleus ATAC-seq (10x multiome with MULTI-seq)
multiplexed sample: Homo sapiens (male) GM25256 (WTC-11) cell line treated with 50 ng/mL interleukin 4 for 1 day; Homo sapiens (male) GM25256 (WTC-11) cell line treated with 100 ng/mL macrophage colony-stimulating factor 1 for 7 days; ... and 13 more samples

Attribution

Award
HG012076
Principal Investigator(s)
Contact P.I.
Ansuman Satpathy
Lab
Ansuman Satpathy, Stanford